STEREOCHEMISTRY OF THE KDO8P SYNTHASE - AN EFFICIENT SYNTHESIS OF THE3-FLUORO ANALOGS OF KDO8P

Authors
KOHEN A BERKOVICH R BELAKHOV V BAASOV T
Citation
A. Kohen et al., STEREOCHEMISTRY OF THE KDO8P SYNTHASE - AN EFFICIENT SYNTHESIS OF THE3-FLUORO ANALOGS OF KDO8P, Bioorganic & medicinal chemistry letters, 3(8), 1993, pp. 1577-1582
Citations number
21
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
3
Issue
8
Year of publication
1993
Pages
1577 - 1582
Database
ISI
SICI code
0960-894X(1993)3:8<1577:SOTKS->2.0.ZU;2-S
Abstract
Direct evidence of the overall stereochemistry of the KD08P synthase r eaction was obtained by using 3-deuterio and 3-fluoro analogues of pho sphoenolpyruvate as alternative substrates and by subsequent H-1 and F -19 NMR analysis of the enzymatic reaction products. The results revea l the si face addition of enzyme-bound phosphoenolpyruvate to the re f ace of the carbonyl of arabinose 5-phosphate.