THE GENOMIC SEQUENCE OF CARDAMINE CHLOROTIC FLECK CARMOVIRUS

The complete genomic sequence of cardamine chlorotic fleck carmovirus (CCFV) has been determined. The genome is a positive-sense ssRNA molec ule 4041 nucleotides in length, and has 47 to 64 % sequence identity w ith turnip crinkle, carnation mottle and melon necrotic spot carmoviru ses. CCFV and these other carmoviruses have four similar open reading frames (ORFs), and CCFV has large regions of amino acid identity in al l of these ORFs with a European isolate of turnip crinkle virus. CCFV, which replicates well in Arabidopsis thaliana, has only been found so far in Australia in the wild perennial brassica Cardamine lilacina.