AN EPITOPE WITHIN THE DNA-BINDING DOMAIN OF THE HERPES-SIMPLEX VIRUS IMMEDIATE-EARLY PROTEIN VMW175 IS CONSERVED IN THE VARICELLA-ZOSTER VIRUS GENE 62 PROTEIN

We have isolated a panel of monoclonal antibodies that recognize the D NA-binding domain of the herpes simplex virus type 1 (HSV-1) immediate early polypeptide Vmw175. The mice used for the fusions had been immu nized with the isolated Vmw175 DNA-binding domain. This had been purif ied from bacteria that carried a phage T7 expression plasmid with the DNA-binding domain coding region. The epitopes recognized by the monoc lonal antibodies were mapped by using a family of truncated versions o f the DNA-binding domain, which had also been expressed in the bacteri al expression system. The monoclonal antibodies divided into at least four different groups according to this mapping. Several of the monocl onal antibodies recognized Vmw175 expressed in infected BHK cells by H SV-1 strain 17 in Western blots. One of them also recognized the corre sponding protein of varicella-zoster virus gene 62. This is further il lustration of the relatedness of the two polypeptides.