EFFECT OF SALIDROSIDE ON CULTURED MYOCARD IAL-CELLS ANOXIA REOXYGENATION INJURIES

The effects of salidroside (p-hydroxyphenethyl glucoside, Sal, first i solated and synthesized in China) on reoxygenation damages were studie d on cultured myocytes from neonatal rat hearts. At least 80% of cells in the form of monolayer contracted spontaneously on cultured 72 h, t hen the cells were used in the contractility experiment. After anoxia 3 h and reoxygenation for 1 h the beating of myocardial cells was slow ed down and the lactate dehydrogenase (LDH) liberated by myocardial ce lls was increased. Electron microscopy of myocardial cells revealed lo calized defects of cell membrane, dilatation of endoplasmic reticulum, and swelling of mitochondria. One h before anoxia, addition of Sal 10 and 30 mug.ml-1 increased the beat rate of myocardial cells, depresse d the release LDH of from myocytes, and the myocardial ultrasturcture was normal during anoxia and reoxygenation. Hence Sal may provide some protective effects on the anoxia/reoxygenation damages upon myocardiu m.