INHIBITORY EFFECTS OF PROTEIN-KINASE-C IN HIBITOR AND CALMODULIN ANTAGONIST ON TUMOR-NECROSIS-FACTOR PRODUCTION BY MOUSE MACROPHAGES

Citation
M. Tian et al., INHIBITORY EFFECTS OF PROTEIN-KINASE-C IN HIBITOR AND CALMODULIN ANTAGONIST ON TUMOR-NECROSIS-FACTOR PRODUCTION BY MOUSE MACROPHAGES, Zhongguo yaoli xuebao, 14(5), 1993, pp. 447-449
Citations number
10
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
ISSN journal
02539756
Volume
14
Issue
5
Year of publication
1993
Pages
447 - 449
Database
ISI
SICI code
0253-9756(1993)14:5<447:IEOPIH>2.0.ZU;2-E
Abstract
Mouse peritoneal macrophages were primed with calcium ionophore calcim ycin 1 mumol.L-1 for 8 h and then elicited by lipopolysaccharides (LPS , 10 ng.ml-1) for 6 h to induce tumor necrosis factor (TNF), measured by cristal violet staining assay using murine fibroblast L929 cells. P rotein kinase C (PKC) inhibitor, 1-(5-isoquinolinesulphonyl)-2-methylp iperazine (H-7) and calmodulin (CaM) antagonist, N-(6-aminohexyl)-5-ch loro-1-naphthalenesulfonamide (W-7) were studied on LPS-induced TNF pr oduction by macrophages. Addition of H-7 (0.31-50 mug.ml-1) and W-7 (1 0-4000 ng.ml-1) simultaneously wit LPS to the culture medium suppresse d LPS-induced TNF production in a concentration-dependent manner. When macrophages were pretreated with W-7 (10 ng.ml-1), LPS-induced TNF pr oduction was completely inhibited at 8 h. These results suggest that T NF production by macrophages may be dependent on the PKC and CaM.