ANALYSIS AND CONFIGURATION ASSIGNMENTS OF THE AMINO-ACIDS IN A PYOVERDINE-TYPE SIDEROPHORE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The amino acid composition of the siderophore pyoverdine Pf244 was det ermined by reversed phase high-performance liquid chromatography (RP-H PLC) of phenylthiocarbamyl derivatives of the acid-hydrolyzed pyoverdi ne. The siderophore contains an acid-labile amino acid, N(delta)-hydro xyornithine. and an amino acid previously unknown in naturally occurri ng systems, L-threo-beta-hydroxyhistidine. Amino acid chirality assign ments were determined by RP-HPLC of the beta-D-glucopyranosyl isothioc yanate tetraacetate derivatives. Reactions with amino acid oxidases es tablished the L-configuration of threo-beta-hydroxyhistidine.