IDENTIFICATION OF LICHEN SUBSTANCES BY A STANDARDIZED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD

A method for the identification of secondary aromatic lichen substance s using high-performance liquid chromatography (HPLC) with reversed-ph ase columns, gradient elution and benzoic and solorinic acids as stand ards has been developed. A retention index (I), calculated from the el ution time of the appropriate peak with reference to the standards, is used in identification. I values are recorded for 331 compounds chrom atographed in this standard system.