COMPLEMENTATION TAGGING OF COOPERATING ONCOGENES IN KNOCKOUT MICE

Gene disruption and overexpression in mice can be combined with provir al tagging to investigate the biochemical pathways in which oncogenes act. The phenomenon of oncogene collaboration permits a focussed genet ic approach analogous to the strategies employed in suppressor screens in invertebrates and yeast. We illustrate here its application to inv estigating the action of the pim kinases in tumorigenesis. However, it should be possible to utilize this strategy for dissecting a much wid er range of biochemical pathways in mammalian systems.