ANALYSIS OF RED-BLOOD-CELL VOLUME DISTRIBUTIONS USING THE ICSH REFERENCE METHOD - DETECTION OF SEQUENTIAL-CHANGES IN DISTRIBUTIONS DETERMINED BY HYDRODYNAMIC FOCUSING

Citation
Ce. Mclaren et al., ANALYSIS OF RED-BLOOD-CELL VOLUME DISTRIBUTIONS USING THE ICSH REFERENCE METHOD - DETECTION OF SEQUENTIAL-CHANGES IN DISTRIBUTIONS DETERMINED BY HYDRODYNAMIC FOCUSING, Clinical and laboratory haematology, 15(3), 1993, pp. 173-184
Citations number
17
Categorie Soggetti
Hematology
ISSN journal
01419854
Volume
15
Issue
3
Year of publication
1993
Pages
173 - 184
Database
ISI
SICI code
0141-9854(1993)15:3<173:AORVDU>2.0.ZU;2-L
Abstract
Automated haematology analysers determine the volume of red blood cell s and provide an erythrocyte volume distribution. For analysis of thes e distributions, the International Council for Standardization in Haem atology (ICSH) has recommended methods for fitting a single reference distribution and assessing its goodness of fit (ICSH, 1990). To evalua te the suitability of the ICSH reference method for routine use with h aematology analysers, we applied the ICSH method to a reference sample group of 71 healthy individuals and to 618 samples collected from 112 patients. Samples were measured using Sysmex NE-8000 haematology anal ysers. Each distribution was doubly truncated to eliminate artefactual frequency counts and tested for goodness of fit to a single lognormal distribution (McLaren, Brittenham & Hasselblad, 1986) and mixtures of lognormal distributions (McLaren et al., 1991). Analysis of data from healthy individuals demonstrated the reproducibility of the ICSH refe rence method. Analysis of data from patients illustrated the ability o f the method to detect sequential changes in distributions, providing a useful tool to monitor the effect of therapeutic intervention and bl ood transfusion.