Ad. Davison et Da. Veal, STORAGE OF A MIXED MICROBIAL CONSORTIUM CAPABLE OF GROWTH ON BIPHENYL, Letters in applied microbiology, 17(3), 1993, pp. 101-103
A mixed microbial consortium, BSEN-2, was isolated from a polychlorina
ted biphenyl-contaminated soil using biphenyl as the sole source of ca
rbon and energy. The community was found to contain nine members based
on colony morphology on plate count agar with biphenyl. BSEN-2 was st
ored on glycerol (15% v/v) at -80-degrees-C and was regenerated 3, 30,
100 and 250 d after freezing. No loss of community members or signifi
cant change in growth kinetics were observed. This method provides a s
imple, convenient and reliable method of producing mixed microbial ino
cula.