A RAPID METHOD FOR THE DETERMINATION OF BACTERIAL FATTY-ACID COMPOSITION

Authors
ROZES N GARBAY S DENAYROLLES M LONVAUDFUNEL A
Citation
N. Rozes et al., A RAPID METHOD FOR THE DETERMINATION OF BACTERIAL FATTY-ACID COMPOSITION, Letters in applied microbiology, 17(3), 1993, pp. 126-131
Citations number
20
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
17
Issue
3
Year of publication
1993
Pages
126 - 131
Database
ISI
SICI code
0266-8254(1993)17:3<126:ARMFTD>2.0.ZU;2-P
Abstract
Fatty acid methyl esters suitable for gas chromatographic analysis wer e prepared by treatment of bacterial cells with sodium methoxide (1 N) in anhydrous methanol. The methanolysis of cells, washed or not with sterile water, was carried out at room temperature for 5 min. This met hod was effective for Gram-positive and Gram-negative bacteria, but no t for growing yeast cells. The coefficient of variation of fatty acid methyl ester values was 4.7% of the mean values (range 0.4-25%).