ISOLATION AND PROPERTIES OF ARABINOFURANOSIDASE FROM GEOTRICHUM-CANDIDUM-3C

Isolation of two homogeneous alpha-L-arabinofuranosidases from the cul ture liquid of Geotrichum candidum 3C with 235-fold purification and 3 3% yield is described The molecular weights of arabinofuranosidase-1 a nd arabinofuranosidase-2 are 80 and 69 kD, and their pI's are 4. 00 an d 3.85, respectively. The kinetic characteristics of the two forms in p-nitrophenyl alpha-L-arabinofuranoside hydrolysis and the pH optimum, pH stability, temperature optimum, and temperature stability have bee n determined