INHIBITION-TEST OF BACTERIAL-GROWTH FOR T HE RESEARCH OF CHEMIOANTIBIOTICS IN EGGS

The use of antimicrobic tools in the zootechnical field causes good an imal growth but may also produce allergic and neurotoxic effects in hu mans following the ingestion of antibiotic residues in flesh, milk and eggs. This practice is responsible for antibiotic-endurance due to mi crorganism contact with sublethal doses of drugs. Since in Italy there is a large consumption of eggs, we have trought to look for some chem ioantibiotics in hen egg yolk. Thus we have adjusted a method which us es an inhibition-test of bacterial growth called (Car-Test Unipath). T his method is able to detect small amounts of chemioantibiotics: 560 n g of oxytetracycline, 750 ng of sulfadimethoxine, 400 ng of sulfametho xipyridazine, 200 ng of spiramicin; moreover, this method isn't affect ed by yolk matrix interference. Therefore we believe that this analyti cal method is suitable for a constant search of antibiotics on samples of eggs available in the shops or taken directly from production.