IMMUNOLOGICAL DETECTION OF BOVINE CASEINOMACROPEPTIDE IN OVINE AND CAPRINE DAIRY-PRODUCTS

Polyclonal antibodies against the 139-152 fragment of bovine kappa-cas ein were produced on rabbit using a synthetic peptide as immunogen. Th ese antibodies are monospecific since they recognize their epitope on the bovine native protein and they do not react with the ovine and cap rine homologous proteins. A competitive enzyme linked immunosorbent as say (ELISA) was then successfully developed for the detection of defin ed amounts of cow milk (0.25 to 64%, v/v) in ovine and caprine milk pr oducts. The heating of bovine milk has no influence on the sensitivity and reproducibility of the assay.