CULTIVAR IDENTIFICATION IN TRITICUM-AESTIVUM USING HIGHLY POLYMORPHICRFLP PROBES

Two probes, specific for HMW-glutenins and gamma-gliadins have been us ed to identify 50 common wheat Italian cultivars, most of which are cl osely related, and four common wheat cultivars originating outside Ita ly. The probes revealed complex polymorphic patterns; three probe/enzy me combinations had the necessary sensitivity for the identification o f all 54 cultivars. As already shown for potato and barley, the use of four-cutter restriction enzymes and polyacrylamide gels proved partic ularly useful for detecting polymorphism.