TYPE-1 PLASMINOGEN-ACTIVATOR INHIBITOR GENE-EXPRESSION FOLLOWING PARTIAL-HEPATECTOMY

Citation
J. Schneiderman et al., TYPE-1 PLASMINOGEN-ACTIVATOR INHIBITOR GENE-EXPRESSION FOLLOWING PARTIAL-HEPATECTOMY, The American journal of pathology, 143(3), 1993, pp. 753-762
Citations number
47
Categorie Soggetti
Pathology
ISSN journal
00029440
Volume
143
Issue
3
Year of publication
1993
Pages
753 - 762
Database
ISI
SICI code
0002-9440(1993)143:3<753:TPIGFP>2.0.ZU;2-R
Abstract
A murine model of partial hepatectomy (PH) was employed to investigate type 1 plasminogen activator inhibitor (PAI-1) gene expression in reg enerating liver. Mice were anesthetized, and a portion of the left lob e of the liver was ligated and resected distal to the ligature, and at various times thereafter, total liver RNA was prepared and analyzed b y Northern blotting. PH caused a transient increase in PAI-1 messenger (m)RNA that was apparent within 1 to 2 hours after surgery, was maxim al at 8 hours (ninefold increase over sham-operated controls), and the n slowly declined. Analysis of discrete liver segments demonstrated mu ch greater induction of PAI-1 mRNA in the region adjacent to PH than i n more distal regions. Further analysis of the adjacent tissue by in s itu hybridization revealed that PAI-1 mRNA was induced primarily in he patocytes in the transition zone created by the occluding hemostatic l igature between viable and necrotic tissue. Expression of PAI-1 mRNA c ould also be detected in this transition zone in capsular mesothelial cells, subcapsular hepatocytes, and venous endothelial cells bordering the area. A much weaker signal was evident in hepatocytes dispersed t hroughout the remaining intact lobes of PH mice, and no signal was det ected in the livers of sham-operated mice. These observations suggest that PAI-1 may be of importance in local tissue remodeling events acco mpanying liver regeneration.