CHARACTERIZATION OF CDNAS ENCODING THE MURINE INTERLEUKIN-2 RECEPTOR (IL-2R) GAMMA-CHAIN - CHROMOSOMAL MAPPING AND TISSUE-SPECIFICITY OF IL-2R GAMMA-CHAIN EXPRESSION
Xq. Cao et al., CHARACTERIZATION OF CDNAS ENCODING THE MURINE INTERLEUKIN-2 RECEPTOR (IL-2R) GAMMA-CHAIN - CHROMOSOMAL MAPPING AND TISSUE-SPECIFICITY OF IL-2R GAMMA-CHAIN EXPRESSION, Proceedings of the National Academy of Sciences of the United Statesof America, 90(18), 1993, pp. 8464-8468
The IL-2R gamma chain (IL-2Rgamma) is an essential component of high-
and intermediate-affinity IL-2Rs, playing critical roles for ligand bi
nding and internalization. Recently, our laboratory has demonstrated t
hat IL-2Rgamma mutation results in X chromosome-linked severe combined
immunodeficiency in humans, suggesting that IL-2Rgamma plays a vital
role in thymic maturation of human T cells. We now report the isolatio
n and characterization of cDNAs encoding murine IL-2Rgamma. The open r
eading frame encodes 369 aa, identical in length to that encoded by th
e human IL-2Rgamma cDNA. Murine IL-2Rgamma and human IL-2Rgamma have 6
9% and 70% identity at the nucleotide and amino acid levels, respectiv
ely. As expected, the murine IL-2Rgamma retains the WSXWS motif and fo
ur cysteine residues characteristic of cytokine receptor superfamily m
embers. IL-2Rgamma mRNA distribution shows significant tissue specific
ity, with particularly high-level expression in spleen and thymus, and
higher expression in single-positive (CD4+8- or CD4-8+)-enriched thym
ocytes than in double-negative (CD4-8-) thymocytes. Finally, we have l
ocalized the murine IL-2Rgamma gene, Il2rg, to the X chromosome betwee
n Rsvp and Plp and demonstrated that a defect in IL-2Rgamma is not res
ponsible for the X chromosome-linked xid mutation, which maps to this
same region. The cloning of the murine IL-2Rgamma cDNA will facilitate
the investigation of the role of this protein in lymphocyte function
and thymic development.