USE OF ISOZYMES TO CHARACTERIZE TRITICUM-AESTIVUM AEGILOPS-MARKGRAFIIADDITION LINES

Sixteen leaf and three seed isozymes were tested for their ability to identify alien chromosomes in Triticum aestivum - Aegilops markgrafii amphiploid and six descending disomic addition lines. Four leaf isozym es (NADP-dependent aromatic alcohol dehydrogenase, aspartate aminotran sferase, esterase, leucine aminopeptidase) and three seed enzymes (alc ohol dehydrogenase, beta-amylase, esterase) from Ae. markgrafii were f ound to be informative in differentiating both the amphiploid and five of the six different addition lines from wheat. Leaf peroxidase, an e ighth informative marker among the 19 isozyme systems tested was only in the amphiploid present, none of the six addition lines showed the A egilops-specific band.