CROSS-HYBRIDIZATION OF EQUID HERPESVIRUS-2 (EHV-2) AND HERPES-SIMPLEXVIRUS-1 (HSV-1) GENES TO EQUID HERPESVIRUS-1 (EHV-1)

Authors
PUREWAL AS SMALLWOOD AV ALLSOPP R WELCH HM EDINGTON N
Citation
As. Purewal et al., CROSS-HYBRIDIZATION OF EQUID HERPESVIRUS-2 (EHV-2) AND HERPES-SIMPLEXVIRUS-1 (HSV-1) GENES TO EQUID HERPESVIRUS-1 (EHV-1), Veterinary microbiology, 35(1-2), 1993, pp. 1-10
Citations number
36
Categorie Soggetti
Microbiology,"Veterinary Sciences
Journal title
Veterinary microbiology → ACNP
ISSN journal
03781135
Volume
35
Issue
1-2
Year of publication
1993
Pages
1 - 10
Database
ISI
SICI code
0378-1135(1993)35:1-2<1:COEH(A>2.0.ZU;2-R
Abstract
In contrast to previous findings, the Ab4 isolate of equid herpesvirus -1 (EHV-1) was shown to share homology with the G9 isolate of equid he rpesvirus-2 (EHV-2). Using Southern blotting and stringent hybridizati on conditions, a significant proportion of this cross-hybridization wa s identified by the immediate-early gene-3 (IE-3) probe from herpes si mplex virus-1 (HSV-1 ), The HSV-1 U(L)48 gene probe (encoding the IE g ene transactivating protein VmW65, which is also known as alpha-TIF or VP16) was used to identify and isolate its counterpart in EHV-1. The relevance of shared homology to transactivation is being investigated.