A COMBINED POLYMERASE CHAIN REACTION-COLOR DEVELOPMENT HYBRIDIZATION ASSAY IN A MICROTITRE FORMAT FOR THE DETECTION OF CLOSTRIDIUM SPP

Authors
GALINDO I RANGELALDAO R RAMIREZ JL
Citation
I. Galindo et al., A COMBINED POLYMERASE CHAIN REACTION-COLOR DEVELOPMENT HYBRIDIZATION ASSAY IN A MICROTITRE FORMAT FOR THE DETECTION OF CLOSTRIDIUM SPP, Applied microbiology and biotechnology, 39(4-5), 1993, pp. 553-557
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
39
Issue
4-5
Year of publication
1993
Pages
553 - 557
Database
ISI
SICI code
0175-7598(1993)39:4-5<553:ACPCRD>2.0.ZU;2-9
Abstract
We have developed a rapid and sensitive assay for the detection of clo stridial cells or spores in liquid food samples. The method recognizes Clostridium 16S (small ribosomal subunit RNA) rDNA (ribosomal DNA) se quences by a polymerase chain reaction-digoxigenin-labelling protocol, coupled to a capture oligonucleotide immobilized on a microtitre plat e. The positive results are revealed by means of a colour reaction. In 6 h of non-intensive labour, we can detect as few as two to five clos tridial cells or spores in experimentally contaminated soft drinks.