Mp. Pons et Mc. Fuste, URANIUM UPTAKE BY IMMOBILIZED CELLS OF PSEUDOMONAS STRAIN EPS-5028, Applied microbiology and biotechnology, 39(4-5), 1993, pp. 661-665
Polyacrylamide-gel-immobilized cells of Pseudomonas strain EPS 5028 we
re effective in the removal of uranium (U) from synthetic effluents. M
etal accumulation was performed in an open system in columns filled wi
th immobilized cells that were challenged with continuous flows contai
ning U. Possible variables of the system were studied. Uranium uptake
by the immobilized cells of this microorganism was affected by pH but
not by temperature or flow rate. In addition, U binding could be inter
preted in terms of the Freundlich adsorption isotherm indicating singl
e-layer adsorption. The feasibility of reusing the immobilized cells w
as suggested after the recovery of U with a solution of 0.1 m sodium c
arbonate.