MEASUREMENT OF INTRACELLULAR IONIZED CALCIUM IN A FREE-LIVING SOIL NEMATODE, CAENORHABDITIS-ELEGANS

Authors
KAWAII S YOSHIZAWA Y MIZUTANI J
Citation
S. Kawaii et al., MEASUREMENT OF INTRACELLULAR IONIZED CALCIUM IN A FREE-LIVING SOIL NEMATODE, CAENORHABDITIS-ELEGANS, Bioscience, biotechnology, and biochemistry, 57(7), 1993, pp. 1115-1118
Citations number
17
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
57
Issue
7
Year of publication
1993
Pages
1115 - 1118
Database
ISI
SICI code
0916-8451(1993)57:7<1115:MOIICI>2.0.ZU;2-H
Abstract
A calcium chelating fluorescence indicator, fura-2, was used to measur e intracellular ionized calcium in Caenorhabditis elegans. The indicat or loading process was harmless to the nematode, and completed within 2-3 h. Fura-2 was loaded mainly at its intestinal tract. The effects o f DOPA on locomotion and the level of intracellular calcium were inves tigated and measured by using a microfluorometer. The addition of DOPA temporarily increased Ca2+!i for several minutes.