A 12.8 KB SEGMENT, ON THE RIGHT ARM OF CHROMOSOME-II FROM SACCHAROMYCES-CEREVISIAE INCLUDING PART OF THE DUR1,2 GENE, CONTAINS 5 PUTATIVE NEW GENES

A 12 820 bp fragment from the right arm of chromosome II of Saccharomy ces cerevisiae was sequenced and analysed. This fragment contains six non-overlapping long open reading frames (ORFs) designated from the ce ntromere- to the telomere-proximal ends as: YBR1441, 1443, 1444, 1445, 1446 and 1448. YBR1441 encodes a polypeptide of 845 amino acids which shares a long consensus domain with products of S. cerevisiae MCM2, M CM3, CDC46 and Schizosaccharomyces pombe cdc21+ genes. These genes are involved in DNA replication. YBR1445 encodes a polypeptide of 404 ami no acids which has strong similarity with the S. cerevisiae KRE2/MNT1, YUR1, KTR1 gene products. The KRE2/MNT1 protein is an alpha-1,2-manno syltransferase. The product of YBR1444, which encodes a protein of 375 amino acids, presents a lipase signature sequence and a peroxisomal t argeting signal. YBR1448, whose sequence extends further on the telome re-proximal end of the fragment, is identical to the 3' end of the DUR 1,2 gene encoding urea amidolyase. The two ORFs, YBR1443 and YBR1446, exhibit no significant similarity with any known gene.