ENANTIOMETRIC SEPARATION WITH USE OF STATIONARY-PHASE COATED WITH MICELLAR BILE-SALT FOR MICROCOLUMN LIQUID-CHROMATOGRAPHY

A novel method for enantiometric separation using an ODS stationary ph ase coated with bile salt micelles in high-performance liquid chromato graphy has been developed, where bile salt surfactants such as sodium cholate and sodium deoxycholate were used for micelle formation. The p resent method was applied to the separation of the enantiomers of 1,1' -binaphthyl-2,2'-diyl hydrogenphosphate (BNDHP) and N-dansylphenylalan ine by using acetonitrile-water solution as the mobile phase. The depe ndence of the capacity factors and separation factors on the acetonitr ile concentration in the mobile phase was examined for BNDHP enantiome rs. These factors decreased with increasing acetonitrile concentration , which resulted in the change of separation characteristics of BNDHP enantiomers. Although BNDHP and N-dansylphenylalanine enantiomers were separated, similar enantiomers such as 2,2'-dihydroxy-1,1'-binaphthyl and other N-dansylamino acids were not separated in the present separ ation system.