IN-VIVO DEPLETION OF CHICKEN T-CELL SUBSETS

In the chicken three types of T-cell receptors can be defined by monoc lonal antibodies TCR1, TCR2 and TCR3, which recognize gammadelta T cel ls, and Vbeta1- and Vbeta2-expressing alphabeta T cells, respectively. In the present report we have analysed means of selectively depleting the gammadelta T cells and the Vbeta1+ alphabeta T cells. Gammadelta T cells, which represent up to 66% of all T cells in blood of a 6-mont h-old chicken, can be effectively depleted by neonatal thymectomy (Tx) to levels as low as 1%. Immunohistology demonstrates a similar deplet ion in lymphoid organs while intestinal epithelium-associated gammadel ta T cells are affected by Tx to a lesser extent. Vbeta1-bearing alpha beta T cells, which comprise about 80% of the alphabeta T cells, were depleted by embryonic and neonatal injection of the TCR2 antibody. In the thymus such treatment depleted only the Vbeta1+ alphabeta T cells with high density expression of T-cell receptor. Therefore, we thymect omized TCR2-treated animals in order to prevent development of mature Vbeta1+ alphabeta T cells from the low density immature thymocytes. Tr eatment of chickens with a total of 22 mg of TCR2 antibody plus Tx red uced Vbeta1+ alphabeta T cells from an average of 65% to 10% of all T cells. In these TCR2 antibody-treated animals the Vbeta2-expressing al phabeta T cells become the predominant type of T cell (average 85%).