SYNTHESIS OF POLYMERIC NEOGLYCOCONJUGATES BASED ON N-SUBSTITUTED POLYACRYLAMIDES

Several types of polymeric glycoconjugates, N-substituted polyacrylami des, have been synthesized by the reaction of activated polymers with omega-aminoalkylglycosides: (i) (carbohydrate-spacer)n-polyacrylamide, 'pseudopolysaccharides'; (ii) acer)n-phosphatidylethanolamine(m)-poly acrylamide, neoglycolipids, derivatives of phosphatidylethanolamine; ( iii) (carbohydrate-spacer)n-biotin(m)-polyacrylamide, biotinylated pro bes; (iv) (carbohydrate-spacer)n-polyacrylamide-(macroporous glass), a ffinity sorbents based on macroporous glass, covalently coated with po lyacrylamide. An almost quantitative yield in the conjugation reaction makes it possible to insert in the conjugate a predetermined quantity of the ligand(s). Pseudopolysaccharides proved to be a suitable form of antigen for activation of polystyrene and poly(vinyl chloride) plat es (ELISA) and nitrocellulose membranes (dot blot), being advantageous over traditional neoglycoproteins. Polyvalent glycolipids insert well in biological membranes: their physical properties, particularly solu bility, can be changed in a desired direction. Biotinylated derivative s were used as probes for detection and analysis of lectins.