S. Sengupta et E. Ali, EFFECT OF TEMPERATURE ON PRONASE CATALYZED ACYLATION OF ALCOHOLS IN ORGANIC-SOLVENTS, Indian journal of chemistry. Sect. B: organic chemistry, including medical chemistry, 36(1), 1997, pp. 37-40
The effect of temperature on the pronase catalysed acylation of alcoho
ls has been studied. The rate of enzyme catalysed acylation of n-propa
nol with p-nitrophenyl acetate in acetonitrile increases almost linear
ly upto the reflux temperature of the solvent. Non-catalysed acylation
upto 56 degrees C is negligible. The acylation of 1, 4-cyclohexanedio
l with acetic, propionic and hexanoic anhydride and p-nitrophenyl acet
ate at different temperatures shows that the optimum temperature for p
ronase catalysed acylation is about 56 degrees C. At higher temperatur
es, there is an increase in the rate of nonenzymatic acylation and enz
yme catalysed hydrolysis of the acylating agent. The hydrolytic reacti
on is particularly rapid at higher temperature in the presence of wate
r at a concentration (1.5% v/v) which is optimum for the enzymatic rea
ction at ambient temperature.