DIFFERENTIAL ACTIVATION OF MICROGLIA AND ASTROCYTES IN ANISOMORPHIC AND ISOMORPHIC GLIOTIC TISSUE

Reactive astrocytes and microglial cells are both involved in the form ation of gliotic tissue. Using immunohistochemical markers, we have co mpared the response of both these cell types after two different kinds of damage in the brain: traumatic injury (anisomorphic gliosis) and n eurotoxic induced lesion (isomorphic gliosis), in two distinct regions of the brain, the cortex and the hippocampus. We show that the time c ourse and the relative contribution of astrocytes and microglial cells differ greatly in the two kinds of lesions. While in anisomorphic gli osis there is little activation of endogenous microglial cells indepen dently of the brain region damaged, these cells contribute in large me asure and for prolonged periods of time to the formation of isomorphic gliotic tissue. Astrocytes are quickly activated at the border of ani somorphic lesions, and after 3 days they already occupy an extensive p ortion of the brain parenchyma. However, after 1 month, they are found restricted to a thin strip at the lesion boundary. In contrast, after an isomorphic lesion, astrocytes become reactive around the site of n euronal cell loss but not at the site of the lesion itself. Only after 2 weeks do they totally invade the damaged region, persisting for at least 1 month. Such differences are observed independently of the brai n region damaged. These results suggest that the cellular, and therefo re the molecular, composition of gliotic tissue depends on the type of insult the CNS has suffered. (C) 1993 Wiley-Liss, Inc.