IN-VIVO GENE DELIVERY - EFFICIENT TRANSFECTION OF T-LYMPHOCYTES IN ADULT MICE

Citation
R. Philip et al., IN-VIVO GENE DELIVERY - EFFICIENT TRANSFECTION OF T-LYMPHOCYTES IN ADULT MICE, The Journal of biological chemistry, 268(22), 1993, pp. 16087-16090
Citations number
21
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
268
Issue
22
Year of publication
1993
Pages
16087 - 16090
Database
ISI
SICI code
0021-9258(1993)268:22<16087:IGD-ET>2.0.ZU;2-M
Abstract
We have demonstrated that a single intraperitoneal injection of cation ic liposomes complexed to a chloramphenicol acetyltransferase (CAT) ge ne expression plasmid can transfect the majority of splenic Thy 1.2+ T lymphocytes, as well as significant numbers of bone marrow-derived he matopoietic cells, in adult mice. CAT activity was detected in the spl een for at least 2 weeks, and there was no evidence of treatment-relat ed toxicity. Some degree of tissue-specific transgene expression was a chieved by varying the cationic lipid used.