THE INSERTION ELEMENT IS6110 IS A USEFUL TOOL FOR DNA-FINGERPRINTING OF MYCOBACTERIUM-BOVIS ISOLATES FROM CATTLE AND GOATS IN SPAIN

Authors
LIEBANA E ARANAZ A DOMINGUEZ L MATEOS A GONZALEZLLAMAZARES P RODRIGUEZFERRI EF DOMINGO M VIDAL D COUSINS D
Citation
E. Liebana et al., THE INSERTION ELEMENT IS6110 IS A USEFUL TOOL FOR DNA-FINGERPRINTING OF MYCOBACTERIUM-BOVIS ISOLATES FROM CATTLE AND GOATS IN SPAIN, Veterinary microbiology, 54(3-4), 1997, pp. 223-233
Citations number
27
Categorie Soggetti
Microbiology,"Veterinary Sciences
Journal title
Veterinary microbiology → ACNP
ISSN journal
03781135
Volume
54
Issue
3-4
Year of publication
1997
Pages
223 - 233
Database
ISI
SICI code
0378-1135(1997)54:3-4<223:TIEIIA>2.0.ZU;2-C
Abstract
A total of 129 Mycobacterium bovis strains from 5 different Spanish lo cations were fingerprinted using the IS6110 repetitive element. We dem onstrated the presence of multiple copies (from 2 to 13) of IS6110 in a large proportion (47.4%) of the M. bovis strains isolated from cattl e and we showed that these strains can be successfully differentiated by means of the RFLP with IS6110. All of the M. bovis strains isolated from goats had multiple copies of IS6110 and 4 bands of 2, 1.7, 1.4 a nd 1.3 kb were common in all the caprine RFLP patterns, The caprine st rains formed a clearly separate cluster from the bovine strains.