Ah. Hammond et Jr. Fry, MAINTENANCE OF XENOBIOTIC METABOLISM AND TOXICITY IN RAT HEPATOCYTE CULTURES AFTER CELL PRESERVATION AT 4-DEGREES, Biochemical pharmacology, 46(2), 1993, pp. 333-335
Isolated rat hepatocytes were stored at 4-degrees in modified Universi
ty of Wisconsin solution for 22-24 hr and then placed into culture. Sa
tisfactory viabilities were obtained for all preparations. No loss of
alcohol dehydrogenase or 7-ethoxy-3,4-dimethylcoumarin 0-deethylase ac
tivity was observed in the cold stored relative to the control culture
s. The protein and glutathione contents, allyl alcohol and precocene I
I (PII) toxicities were also similar, as was the PII-mediated glutathi
one depletion. For the parameters measured, cultures of cold stored he
patocytes were identical to normal cultures.