INHIBITION-KINETICS OF BRAIN BUTYRYLCHOLINESTERASE BY CD2+ AND ZN2+, CA2+ OR MG2+ REACTIVATES THE INHIBITED ENZYME

Authors
COKUGRAS AN TEZCAN EF
Citation
An. Cokugras et Ef. Tezcan, INHIBITION-KINETICS OF BRAIN BUTYRYLCHOLINESTERASE BY CD2+ AND ZN2+, CA2+ OR MG2+ REACTIVATES THE INHIBITED ENZYME, International Journal of Biochemistry, 25(8), 1993, pp. 1115-1120
Citations number
21
Categorie Soggetti
Biology
Journal title
International Journal of Biochemistry → ACNP
ISSN journal
0020711X
Volume
25
Issue
8
Year of publication
1993
Pages
1115 - 1120
Database
ISI
SICI code
0020-711X(1993)25:8<1115:IOBBBC>2.0.ZU;2-4
Abstract
1. The inhibition kinetics of sheep brain butyrylcholinesterase (BChE) (acylcholine acylhydrolase, EC 3.1.1.8) by Cd2+ and Zn2+ has been stu died. 2. K(S) has been determined as 0.14 mM. Cd2+ and Zn2+ were the h yperbolic mixed-type inhibitors of BChE. Ca2+ and Mg2+ had no effect o n the enzyme activity in the experimental conditions. 3. But when the enzyme was inhibited by 0.1 mM Cd2+ or Zn2+, Ca2+ and Mg2+ reactivated the inhibited form of BChE.