CLONING OF PROTOCATECHUATE 3,4-DIOXYGENASE GENES FROM BRADYRHIZOBIUM-JAPONICUM USDA110

Authors
PODILA GK KOTAGIRI S SHANTHARAM S
Citation
Gk. Podila et al., CLONING OF PROTOCATECHUATE 3,4-DIOXYGENASE GENES FROM BRADYRHIZOBIUM-JAPONICUM USDA110, Applied and environmental microbiology, 59(8), 1993, pp. 2717-2719
Citations number
19
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
59
Issue
8
Year of publication
1993
Pages
2717 - 2719
Database
ISI
SICI code
0099-2240(1993)59:8<2717:COP3GF>2.0.ZU;2-B
Abstract
A heterologous gene probe encoding the alpha and beta subunits of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase (PCD) was used to detect its homolog in the genome of Bradyrhizobium japonicum USDA110. Three cosmid clones carrying a 2.2-kb BamHI insert showed high levels of PCD activity. SacI digestion of one of the genomic clones, pBjG17, produced a 2.5-kb insert DNA that complemented a PCD mutant of P. cepa cia.