Nk. Maiti et al., AN IMPROVED DOT ELISA TO DETECT FOWL ADENOVIRUS TYPE-1 ANTIGEN, Comparative immunology, microbiology and infectious diseases, 16(3), 1993, pp. 245-250
An improved dot immunobinding assay to detect fowl adenovirus type-1 i
s described. The method consists of spotting of antigen on nitrocellul
ose membrane sheet, blocking with either 5% acetic acid or with 5% def
atted milk powder and fixation of either antigen or antigen-antibody c
omplex, with either 50% methanol or 0.25% glutaraldehyde or 0.2% tanni
c acid. The results revealed that fixation of both antigen and antigen
antibody complex resulted in 4-fold increase in sensitivity when acet
ic acid was used as blocking agent. The use of two substrates simultan
eously resulted in more colour intensity and clarity than using both s
ubstrates separately.