AN IMPROVED DOT ELISA TO DETECT FOWL ADENOVIRUS TYPE-1 ANTIGEN

An improved dot immunobinding assay to detect fowl adenovirus type-1 i s described. The method consists of spotting of antigen on nitrocellul ose membrane sheet, blocking with either 5% acetic acid or with 5% def atted milk powder and fixation of either antigen or antigen-antibody c omplex, with either 50% methanol or 0.25% glutaraldehyde or 0.2% tanni c acid. The results revealed that fixation of both antigen and antigen antibody complex resulted in 4-fold increase in sensitivity when acet ic acid was used as blocking agent. The use of two substrates simultan eously resulted in more colour intensity and clarity than using both s ubstrates separately.