The accumulation of metal ions on to biological materials is a promisi
ng method for removing these toxic materials from waste or ground-wate
rs. A fractional factorial statistical experimental design was used to
ascertain which experimental parameters affect the sorption of Cr6+ b
y a consortium of denitrifying bacteria using a minimum number of expe
riments. Analysis of this set of experiments found that the steady sta
te sorption behavior could be described by Langmuir isotherms. Observe
d variations in the isotherm parameters were due to the imposed change
s in the experimental variables. In particular, solution pH and the st
ate of the biomass were found to have the greatest influence upon the
amount of chromium that was taken up, while other factors had less sig
nificance. but were still important.