REDUCED TUMORIGENICITY AND CELL MOTILITY OF A GASTRIC-CARCINOMA CELL-LINE BY INTRODUCTION OF WILD-TYPE P53 GENE

Wild-type or mutant human p53 gene was transfected into a human gastri c carcinoma cell line MKN-1 which shares a mutant p53 allele. Transfec ted wild-type p53 reduced the colony forming efficiency and tumorigeni city of MKN-1 cells. However, no difference in expression of cell adhe sion molecule, oncogenes and growth factors was observed among parent, wild-type p53 and mutant p53 transfectants. In motility assay, the wi ld-type p53 transfectants relative to the parental or mutant p53 trans fectants exhibited a decreased motility, and HGF had a greater effect on the motility of the mutant p53 transfectants, but very little effec t on the motility of either the parental or wild-type transfectants. I n invasion assay, mutant p53 transfectants revealed the increased inva sion ability into collagen gel. These results suggest that allele loss and point mutation of p53 gene may play a critical role not only in g rowth but also in invasion of gastric carcinoma cells.