CLONING AND SEQUENCING OF THE GLUCOSYL TRANSFERASE-ENCODING GENE FROMCONVERTING BACTERIOPHAGE X (SFX) OF SHIGELLA-FLEXNERI

Authors
VERMA NK VERMA DJ HUAN PT LINDBERG AA
Citation
Nk. Verma et al., CLONING AND SEQUENCING OF THE GLUCOSYL TRANSFERASE-ENCODING GENE FROMCONVERTING BACTERIOPHAGE X (SFX) OF SHIGELLA-FLEXNERI, Gene, 129(1), 1993, pp. 99-101
Citations number
15
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
129
Issue
1
Year of publication
1993
Pages
99 - 101
Database
ISI
SICI code
0378-1119(1993)129:1<99:CASOTG>2.0.ZU;2-2
Abstract
Shigella flexneri type Y strains (-;3,4) are converted to type X (-;7, 8) by bacteriophage X (SFX) that causes glucosylation of the O-antigen ic polysaccharide chain. The gene (gtr) encoding glucosyl transferase from bacteriophage X has been cloned and sequenced. The protein encode d by gtr consists of 416 amino acids with a M(r) of 47 369. The cloned gtr product was able to convert a S. flexneri strain type Y (SFL 124, a live attenuated candidate vaccine strain) to type X. The importance of the hybrid strain in vaccine development is discussed.