THE CLEAVAGE SITES AND LOCALIZATION OF GENES ENCODING THE RESTRICTIONENDONUCLEASES ECO1831I AND ECOHI

Authors
KRAVETZ AN ZAKHAROVA MV BELETSKAYA IV SINEVA EV DENJMUCHAMETOV MM PETROV SI GLATMAN LI SOLONIN AS
Citation
An. Kravetz et al., THE CLEAVAGE SITES AND LOCALIZATION OF GENES ENCODING THE RESTRICTIONENDONUCLEASES ECO1831I AND ECOHI, Gene, 129(1), 1993, pp. 153-154
Citations number
11
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
129
Issue
1
Year of publication
1993
Pages
153 - 154
Database
ISI
SICI code
0378-1119(1993)129:1<153:TCSALO>2.0.ZU;2-5
Abstract
The restriction endonucleases Eco1831I and EcoHI cleave before the fir st 5'-cytosine in the recognition sequence 5' - down CCSGG - - 3'/3' - - GGSCC up - 5' (where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA+ h osts. Thus, they differ in their cleavage specificity from that of the BcnI isoschizomer, which cleaves after the second 5' cytosine.