MONOCLONAL-ANTIBODY TO N-ACETYLGLUCOSAMIN L-(BETA-1-4)-N-ACETYLMURAMYL-ALANYL-D-ISOGLUTAMINE - PREPARATION AND CHARACTERIZATION

Authors
MAREEVA TY KOTOVA OV MAKAROV EA ANDRONOVA TM NESMEYANOV VA
Citation
Ty. Mareeva et al., MONOCLONAL-ANTIBODY TO N-ACETYLGLUCOSAMIN L-(BETA-1-4)-N-ACETYLMURAMYL-ALANYL-D-ISOGLUTAMINE - PREPARATION AND CHARACTERIZATION, Bioorganiceskaa himia, 19(5), 1993, pp. 555-561
Citations number
19
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
19
Issue
5
Year of publication
1993
Pages
555 - 561
Database
ISI
SICI code
0132-3423(1993)19:5<555:MTNL>2.0.ZU;2-7
Abstract
Hybridoma E6/1.2 was produced by fusion of splenocytes from mice immun ized with yl-(beta1-4)-N-acetylmuramyl-alanyl-D-isoglutamine (GMDP), c onjugated to methylated BSA, with SP2/0 myeloma cells. GMDP-specific m onoclonal antibody was IgGl subtype and had affinity constant 2.10(9) M-1. According to competitive ELISA, the presence of the intact disacc haride fragment and the alanyl residue was critical for the GMDP-antib ody interaction.