USE OF CYCLODEXTRINS AS CHIRAL SELECTORS FOR DIRECT RESOLUTION OF THEENANTIOMERS OF FLUOXETINE AND ITS METABOLITE NORFLUOXETINE BY HPLC

The goal of this work is to investigate the direct chromatographic sep aration of the enantiomers of fluoxetine and its active metabolite nor fluoxetine. The liquid chromatographic retention behavior of these ena ntiomers on a beta-cyclodextrin bonded-phase column was investigated w ith respect to mobile phase composition, pH, ionic strength, and solve nt selectivity. Relationships were established between these factors a nd the three most important chromatographic parameters: retention time , resolution, and selectivity. Most of the evidence suggests that the unique selectivity of this column isdue to inclusion complex formation , which provides the physical basis for enantiomeric resolution. After these studies a set of optimum chromatographic conditions was chosen for the simultaneous separation/determination of a mixture of the four enantiomers using fluorescence detector. (C) 1993 Wiley-Liss, Inc.