S. Piperaki et M. Parissipoulou, USE OF CYCLODEXTRINS AS CHIRAL SELECTORS FOR DIRECT RESOLUTION OF THEENANTIOMERS OF FLUOXETINE AND ITS METABOLITE NORFLUOXETINE BY HPLC, Chirality, 5(4), 1993, pp. 258-266
The goal of this work is to investigate the direct chromatographic sep
aration of the enantiomers of fluoxetine and its active metabolite nor
fluoxetine. The liquid chromatographic retention behavior of these ena
ntiomers on a beta-cyclodextrin bonded-phase column was investigated w
ith respect to mobile phase composition, pH, ionic strength, and solve
nt selectivity. Relationships were established between these factors a
nd the three most important chromatographic parameters: retention time
, resolution, and selectivity. Most of the evidence suggests that the
unique selectivity of this column isdue to inclusion complex formation
, which provides the physical basis for enantiomeric resolution. After
these studies a set of optimum chromatographic conditions was chosen
for the simultaneous separation/determination of a mixture of the four
enantiomers using fluorescence detector. (C) 1993 Wiley-Liss, Inc.