HLA-DR AND HLA-DQ GENOTYPING BY PCR-SSO IN SHANGHAI CHINESE

The polymorphism of HLA class II (HLA-DRB1, -DQA1 and -DQB1) were inve stigated in 89 Shanghai Han Chinese using polymerase-chain reaction am plification and oligonucleotide (PCR-SSO) typing. Of the 43 DRB1 allel es tested, 24 were observed. DRB10901 (16.85%), *0803 (9.55%), *1202 (9.55%) and 1501 (12.92%) were most frequent and account for the high frequencies of DR9, DR8, DR5 and DR2 specificities in this population . The alleles DRB10101, *0102 and *1001 had very low frequencies. Amo ng the 8 DQA1 and 13 DQB1 alleles tested, only DQA10401 and DQB1*0402 were absent. DQA10301, and DQB1*0301 and *0303 were among the most c ommon alleles of the two loci respectively. Unusual DRB1-DQA1-DQB1 hap lotypes were observed and a DRB1 ''new'' allele was suggested.