Purpose: To evaluate the effect of four proprietary dentin desensitizi
ng agents on dentin tubular occlusion, chemical composition changes on
the dentin surface, and the effect of saliva and toothbrushing on the
se agents. Materials and Methods: Fifty dentin discs, obtained from 50
freshly extracted human premolar and molar teeth were used in this st
udy. These were divided into five groups of 10 discs each. Five discs
from each group were treated with the desensitizing agents, viewed und
er the SEM and subjected to energy dispersive X-ray analysis. The othe
r five discs were treated with the desensitizing agents, immersed in a
rtificial saliva, subjected to simulated toothbrushing equivalent to 3
weeks of normal brushing and viewed under the SEM. The agents studied
were Sensodyne Dentin Desensitizer, Therma-Trol Desensitizer Gel, Glu
ma Desensitizer and All-Bond DS. Results: The Kruskal-Wallis test foll
owed by the Wilcoxon signed-rank test showed that Sensodyne Dentin Des
ensitizer exhibited the greatest amount of tubular occlusion among the
unbrushed samples, followed by Therma-Trol Desensitizer Gel, Gluma De
sensitizer and All-Bond DS (P < 0.05) in that order. Toothbrushing inc
reased tubular occlusion in all cases except the Sensodyne Dentin Dese
nsitizer treated samples.