ANALYSIS OF THE ESCHERICHIA-COLI GENOME .3. DNA-SEQUENCE OF THE REGION FROM 87.2 TO 89.2 MINUTES

The DNA sequence of 96.5 kilobases of the Escherichia coli K-12 genome has been determined, spanning the region between rrna at 87.2 minutes and katG at 89.2 minutes on the genetic map. The sequence includes 84 open reading frames, of which 46 code for unidentified proteins. Six previously mapped but unsequenced genes have been identified in this s pan: mob, fdhD, rhaD, rhaA, rhaB, and kdgT. In addition, five new gene s have been assigned: the heat shock genes hslU and hslV, and the gene s fdoG, fdoH, and fdol, which encode the three subunits of formate deh ydrogenase-O. The arrangement of the genes relative to possible promot ers and terminators suggests 57 potential transcription units. Other f eatures include the precise location of the bacteriophage P2 attachmen t site attP211, and eleven REP elements, including one containing 9 RE P sequences-one of the largest such elements known. This segment bring s the total length of contiguous finished sequence to 325 kilobases.