Sl. Swenson et al., DIAGNOSTIC COMPATIBILITY OF A THYMIDINE KINASE, INVERTED REPEAT, GI, AND GPX MODIFIED-LIVE GENE-DELETED PRV VACCINE WITH 3 DIFFERENTIAL ELISAS, Journal of veterinary diagnostic investigation, 5(3), 1993, pp. 347-350
The differential pseudorabies virus (PRV) vaccines currently in use in
the USA have deletions of the genes coding for the glycoprotein I (gI
) and/or glycoprotein X (gpX). The absence of gI and/or gpX allow for
the serologic differentiation of vaccinated swine from PRV-infected sw
ine using differential enzyme-linked immunosorbent assays (ELISAs). A
newly developed pseudorabies vaccine virus has 4 deletions of the vira
l genome: the genes coding for gI, gpX, and thymidine kinase and a por
tion of the repeat region to attenuate the virus. The purpose of this
work was to evaluate the diagnostic compatibility of the gI/gpX gene-d
eleted vaccine with 3 differential vaccines and 3 differential ELISAs
currently in use. Pigs vaccinated 3 times with the gI/gpX gene-deleted
vaccine remained seronegative on the 3 differential ELISAs tested. Pi
gs previously vaccinated with either a gI or gpX gene-deleted vaccine
and then vaccinated with the gI/gpX gene-deleted vaccine remained sero
negative on the respective gI or gpX differential assay.