DEVELOPMENT AND EVALUATION OF A MIXED-ANTIGEN ELISA FOR SERODIAGNOSISOF ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPES 1, 5, AND 7 INFECTIONS IN COMMERCIAL SWINE HERDS

Authors
BOSSE JT FRIENDSHIP R ROSENDAL S FENWICK BW
Citation
Jt. Bosse et al., DEVELOPMENT AND EVALUATION OF A MIXED-ANTIGEN ELISA FOR SERODIAGNOSISOF ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPES 1, 5, AND 7 INFECTIONS IN COMMERCIAL SWINE HERDS, Journal of veterinary diagnostic investigation, 5(3), 1993, pp. 359-362
Citations number
NO
Categorie Soggetti
Veterinary Sciences
Journal title
Journal of veterinary diagnostic investigation → ACNP
ISSN journal
10406387
Volume
5
Issue
3
Year of publication
1993
Pages
359 - 362
Database
ISI
SICI code
1040-6387(1993)5:3<359:DAEOAM>2.0.ZU;2-M
Abstract
A mixed-antigen enzyme-linked immunosorbent assay (ELISA) containing s erotype-specific polysaccharide antigens from serotypes 1, 5, and 7 of Actinobacillus pleuropneumoniae was developed. This ELISA was evaluat ed using sera from experimentally infected pigs. With a negative cutof f value of 0.250 (optical density at 405 nm), sensitivity and specific ity were determined to be 96% and 99.5%, respectively. The ELISA was f urther evaluated using sera from commercial swine. Sows and suckling p iglets were found to be the best age groups for detection of positive reactors to A. pleuropneumoniae. The mixed-antigen ELISA could be used in a herd health monitoring program for detection of A. pleuropneumon iae infections.