Nd. Cohen et al., GENUS-SPECIFIC DETECTION OF SALMONELLAE USING THE POLYMERASE CHAIN-REACTION (PCR), Journal of veterinary diagnostic investigation, 5(3), 1993, pp. 368-371
Oligonucleotide primers for the polymerase chain reaction (PCR) that e
nable genus-specific detection of members of the genus Salmonella were
developed. The primers amplify a 496-bp genetic sequence of members o
f the genus Salmonella. Amplification of DNA extracted from all other
genera of the family Enterobacteriaceae and various other gram-positiv
e aerobic and anaerobic bacteria yielded negative results. Application
s of the PCR using these genus-specific primers are discussed.