ESSENTIAL OIL PRODUCTION BY ANTHEMIS-NOBILIS L TISSUE-CULTURE

The production of essential oil by tissue cultures of Anthemis nobilis L. including cell suspension, shoot and crown-gall cultures is report ed. The biosynthetic capability of these different cultures was compar ed with that of plants grown in a field. The addition of crude polysac charide fraction prepared from yeast extract and from the plant itself to shoot cultures affected the composition and total essential conten t (from 0.08% to 0.30% dry weight). The essential oil content of the c rown-gall tissue of A. nobilis was 0.25% of the dry weight and the com position of the essential oil was comparable to that of the flowers.