SCREENING OF SYNTHETIC PEPTIDE LIBRARIES WITH RADIOLABELED ACCEPTOR MOLECULES

A method has been developed for the identification of specific accepto r molecule-binding sequences from a chemically synthesized peptide lib rary. The peptide resin beads, each bead carrying one peptide sequence , are incubated with radiolabeled acceptor molecule and subsequently i mmobilized in a thin layer of agarose. Resin beads that carry acceptor molecule-specific sequences are identified by autoradiography and sub jected to automated gas-phase sequencing. The method was tested by scr eening a synthetic pentapeptide library with an anti-beta-endorphin mo noclonal antibody.