IDENTIFICATION OF STRUCTURAL NITROGEN-FIXATION (NIF) GENES IN BACILLUS-POLYMYXA AND BACILLUS-MACERANS

Authors
OLIVEIRA SS SELDIN L BASTOS MCF
Citation
Ss. Oliveira et al., IDENTIFICATION OF STRUCTURAL NITROGEN-FIXATION (NIF) GENES IN BACILLUS-POLYMYXA AND BACILLUS-MACERANS, World journal of microbiology & biotechnology, 9(3), 1993, pp. 387-389
Citations number
NO
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
World journal of microbiology & biotechnology → ACNP
ISSN journal
09593993
Volume
9
Issue
3
Year of publication
1993
Pages
387 - 389
Database
ISI
SICI code
0959-3993(1993)9:3<387:IOSN(G>2.0.ZU;2-Y
Abstract
Some naturally occurring Nif+ and Nif- strains of Bacillus polymyxa an d Bacillus macerans were tested for DNA homology to the Klebsiella pne umoniae nif genes by Southern hybridization. Genomic DNA from all Nif strains carried homologous sequences only to structural nif genes. Th e homology detected was limited to nifH and niFD. The hybridization pa ttern observed suggested that both genes are contiguous on the chromos ome of the Bacillus strains. No homology was found between the genomic DNA from Nif- strains and the K. pneumoniae nif genes.