GENETIC-FACTORS INFLUENCING REGENERATION ABILITY IN RYE (SECALE-CEREALE L) .1. IMMATURE INFLORESCENCES

Immature inflorescences of ten rye inbred lines (inbred degree S10 and S11) were cultured on solidified MS medium supplemented with 3.0 mg/d m3 of 2,4-D. According to their capability for callus production expla nts were classified into two groups: responsive (giving weak or intens ive callus production) and non responsive (lack of callus formation). After transferring responsive material into hormone-free medium the re generation of roots or shoots from the intensive growing callus was ob served. Consistent differences between lines in the portion of explant s with a certain response were found. They were divided into five grou ps reacting in the same way. Lines with different in-vitro response we re crossed in an incomplete diallel. F1, F2 and F3 generations were an alyzed and the following conclusions drawn: the ability for plant rege neration from immature inflorescences in rye is determined by numerous loci, has a recessive character, and both callus production and regen eration suppression may be controlled by complementary genes.